Ukrainian forces have launched an operation against pro-Russian activists who seized a police station on Saturday, the interior minister says.
Arsen Avakov announced on his Facebook page that "all security units" were involved in an "anti-terror operation" in the eastern city of Sloviansk.
Russia warned earlier that any use of force in eastern Ukraine could scupper crisis talks due later this week.
The US accuses Moscow of inciting the trouble. The Kremlin denies the charge.
[caption id="" align="alignnone" width="624"] Ukrainian forces used military helicopters in the their bid to shift the Sloviansk protesters[/caption]
Russia's Foreign Minister Sergei Lavrov said the Kiev government was "demonstrating its inability to take responsibility for the fate of the country".
But the US said there had been a "concerted campaign" by forces with Russian support to undermine the authorities in Kiev.
US Secretary of State John Kerry warned of "additional consequences" if Russia failed to make efforts to "de-escalate" and pull its troops back from Ukraine's border.
Four-party talks involving Ukraine, Russia, the US and the EU are due to start in Geneva on Thursday.
'Shooting to kill'
On Saturday, armed men took over police stations and official buildings in Sloviansk and two other eastern towns - Kramatorsk and Druzhkovka.
Similar reports emerged from Sloviansk and Kramatorsk of armed men dressed in camouflage arriving in buses and storming the police stations.
[caption id="" align="alignnone" width="624"] Pro-Russian activists have blockaded the security services' office in Luhansk[/caption]
[caption id="" align="alignnone" width="624"] In the regional capital, Donetsk, several public buildings have been occupied Pro-Russian demonstrators also continued their occupation of the main administrative building in the regional capital Donetsk, which they have held for one week.[/caption]
In the regional capital, Donetsk, several public buildings have been occupied
Pro-Russian demonstrators also continued their occupation of the main administrative building in the regional capital Donetsk, which they have held for one week.
A protest leader told the BBC that the activists in Sloviansk took action to support the Donetsk sit-in.
Mr Avakov labelled the actions a "display of aggression by Russia".
Announcing the operation to clear the activists, he warned people to stay in their homes in Sloviansk.
"The separatists are shooting to kill without warning against the approaching special forces," he said,
Local governor Sergei Taruta said "all legitimate means" would be used to combat "acts of terror" and restore order across Donetsk region.
Unconfirmed eyewitnesses accounts suggested there was a gun-battle raging around Sloviansk police station.
Accounts on Ukrainian media said the police station had been recaptured, but officials have not yet commented.
Eastern Ukraine has a large Russian-speaking population and has seen a series of protests since the ousting of Ukraine's pro-Russian President Viktor Yanukovych in February.